Enzymatic Assay ofd-Xylose Isomerase withd-Sorbitol Dehydrogenase
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چکیده
منابع مشابه
Comparing the xylose reductase/xylitol dehydrogenase and xylose isomerase pathways in arabinose and xylose fermenting Saccharomyces cerevisiae strains
BACKGROUND Ethanolic fermentation of lignocellulosic biomass is a sustainable option for the production of bioethanol. This process would greatly benefit from recombinant Saccharomyces cerevisiae strains also able to ferment, besides the hexose sugar fraction, the pentose sugars, arabinose and xylose. Different pathways can be introduced in S. cerevisiae to provide arabinose and xylose utilisat...
متن کاملComparison of the xylose reductase-xylitol dehydrogenase and the xylose isomerase pathways for xylose fermentation by recombinant Saccharomyces cerevisiae
BACKGROUND Two heterologous pathways have been used to construct recombinant xylose-fermenting Saccharomyces cerevisiae strains: i) the xylose reductase (XR) and xylitol dehydrogenase (XDH) pathway and ii) the xylose isomerase (XI) pathway. In the present study, the Pichia stipitis XR-XDH pathway and the Piromyces XI pathway were compared in an isogenic strain background, using a laboratory hos...
متن کاملDirected evolution of xylose isomerase for improved xylose
5 Sun-Mi Lee, Taylor Jellison, and Hal S. Alper* 6 7 8 1 Department of Chemical Engineering, The University of Texas at Austin,1 University Station, 9 C0400,Austin, Texas 78712 10 11 2 Clean Energy Research Center, Korea Institute of Science and Technology, 39-1 Hawolgok12 dong, Seongbuk-gu, Seoul 136-791, Korea 13 14 3 Institute for Cellular and Molecular Biology, The University of Texas at Au...
متن کاملEngineering the substrate specificity of xylose isomerase.
Xylose isomerase (XI) catalyzes the isomerization and epimerization of hexoses, pentoses and tetroses. In order to clarify the reasons for the low reaction efficiency of a pentose sugar, L-arabinose, we determined the crystal structure of Streptomyces rubiginosus XI complexed with L-arabinose. The crystal structure revealed that, when compared with D-xylose and D-glucose, L-arabinose binds to t...
متن کاملEnzymatic assay of D-glucuronate using uronate dehydrogenase.
D-Glucuronate is a key metabolite in the process of detoxification of xenobiotics and in a recently constructed synthetic pathway to produce D-glucaric acid, a "top value-added chemical" from biomass. A simple and specific assay of D-glucuronate would be useful for studying these processes, but existing assays are either time-consuming or nonspecific. Using uronate dehydrogenase cloned from Agr...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1974
ISSN: 0002-1369
DOI: 10.1080/00021369.1974.10861454